CryoEM Workshop 冷凍電顯工作坊

Thursday 28 Mar 2024, 09:00 → 17:00 Asia/Taipei

BHSS, Academia Sinica

The overall goal of Academia Sinica Cryo-EM Facility (ASCEM) is to increase the energy of fundamental life science research of Taiwan and Academia Sinica via introduction of cutting-edge instruments and to assist analyzing protein structure and cellular ultrastructure for further solutions of bio-technology issues and serious disease problems.

This promotional workshop, will be held on Thursday, 28 March 2024, aims to provide a concise and comprehensive perspective on the cryo-electron microscope (cryo-EM) technology and its applications in biological researches. Not only from a scientific point of view, we also will present it in practice. The topics range from cryo-sample preparation, the most critical step to obtain a high-resolution cryoEM structure, EM operations and data collection to data processing. The lectures will be presented in Chinese (全程以中文演講及授課).

In order to ensure a better practical learning experience, the afternoon hands-on session only enroll 15 participants this time. We encourage those with a strong motivation in applying cryo-EM for biological field to seize the opportunity to get up close and personal with ASCEM managers. Learning, experience sharing, or technical consultation and so on are all welcome.

Organizer:

Academia Sinica CryoEM Facility

Contact:

Dr. Yuan-Chih Chang (kondo@gate.sinica.edu.tw)

Ms. Hsin-Hua Lin (linhh@gate.sinica.edu.tw)

09:00 → 10:30 Lecture Session

09:00 Opening remarks

Speaker: Dr Shang-Te Danny Hsu (Institute of Biological Chemistry, Academia Sinica)

09:10 Cryo-EM applications for biomolecular structures

Speaker: Dr Kuen-Phon Wu (Institute of Biological Chemistry, Academia Sinica)

10:00 Uncover the special technique of cryo-EM for biological studies

Speaker: Dr Yuan-Chih Chang (Institute of Biological Chemistry, Academia Sinica)

10:30 → 10:45 Coffee Break

10:45 → 12:00 Lecture Session

10:45 Data Processing - How to use DICOS?

Speaker: Mr Tsung-Hsun Wu (Academia Sinica Cryo-EM Facility)

11:00 Data collection and data processing - CryoSPARC live and CryoSPARC

Speaker: Dr Chun-Hsiung Wang (Academia Sinica Cryo-EM Facility)

12:00 → 13:30 Lunch

13:30 → 14:00 Hands-on Session (3 groups, 5 trainees per group)

13:30 PART 1

Group 1: Negative stain and Cryo-EM grid preparation (Dr. Yuan-Chih Chang)
Group 2: Cryo-ET data collection demonstration on Talos (Dr. Chun-Hsiung Wang)
Group 3: SPA data collection demonstration on Titan combined with CryoSPARC live (Dr. Irene Deli)

14:00 → 14:10 Coffee Break

14:10 → 14:40 Hands-on Session (3 groups, 5 trainees per group)

14:10 PART 2

Group 1: Cryo-ET data collection demonstration on Talos (Dr. Yuan-Chih Chang)
Group 2: SPA data collection demonstration on Titan combined with CryoSPARC live (Dr. Chun-Hsiung Wang)
Group 3: Negative stain and Cryo-EM grid preparation (Dr. Irene Deli)

14:40 → 14:50 c

14:50 → 15:20 Hands-on Session (3 groups, 5 trainees per group)

14:50 PART 3

Group 1: SPA data collection demonstration on Titan combined with CryoSPARC live (Dr. Yuan-Chih Chang)
Group 2: Negative stain and Cryo-EM grid preparation (Dr. Chun-Hsiung Wang )
Group 3: Cryo-ET data collection demonstration on Talos (Dr. Irene Deli)

15:20 → 15:30 c

15:30 → 17:00 Hands-on Session (3 groups, 5 trainees per group)

15:30 Open Discussion and/or Consultation