CryoEM Workshop 冷凍電顯工作坊

Asia/Taipei
BHSS, Academia Sinica

BHSS, Academia Sinica

Description

The overall goal of Academia Sinica Cryo-EM Facility (ASCEM) is to increase the energy of fundamental life science research of Taiwan and Academia Sinica via introduction of cutting-edge instruments and to assist analyzing protein structure and cellular ultrastructure for further solutions of bio-technology issues and serious disease problems.

This promotional workshop, will be held on Thursday, 28 March 2024, aims to provide a concise and comprehensive perspective on the cryo-electron microscope (cryo-EM) technology and its applications in biological researches. Not only from a scientific point of view, we also will present it in practice. The topics range from cryo-sample preparation, the most critical step to obtain a high-resolution cryoEM structure, EM operations and data collection to data processing. The lectures will be presented in Chinese (全程以中文演講及授課).

In order to ensure a better practical learning experience, the afternoon hands-on session only enroll 15 participants this time. We encourage those with a strong motivation in applying cryo-EM for biological field to seize the opportunity to get up close and personal with ASCEM managers. Learning, experience sharing, or technical consultation and so on are all welcome.

Organizer:

Academia Sinica CryoEM Facility

Contact:

Dr. Yuan-Chih Chang (kondo@gate.sinica.edu.tw)

Ms. Hsin-Hua Lin (linhh@gate.sinica.edu.tw)

    • Lecture Session Conf. Room 1

      Conf. Room 1

      BHSS, Academia Sinica

      • 1
        Opening remarks Conf. Room 1

        Conf. Room 1

        BHSS, Academia Sinica

        Speaker: Dr Shang-Te Danny Hsu (Institute of Biological Chemistry, Academia Sinica)
      • 2
        Cryo-EM applications for biomolecular structures Conf. Room 1

        Conf. Room 1

        BHSS, Academia Sinica

        Speaker: Dr Kuen-Phon Wu (Institute of Biological Chemistry, Academia Sinica)
      • 3
        Uncover the special technique of cryo-EM for biological studies Conf. Room 1

        Conf. Room 1

        BHSS, Academia Sinica

        Speaker: Dr Yuan-Chih Chang (Institute of Biological Chemistry, Academia Sinica)
    • 10:30 AM
      Coffee Break
    • Lecture Session Conf. Room 1

      Conf. Room 1

      BHSS, Academia Sinica

      • 4
        Data Processing - How to use DICOS? Conf. Room 1

        Conf. Room 1

        BHSS, Academia Sinica

        Speaker: Mr Tsung-Hsun Wu (Academia Sinica Cryo-EM Facility)
      • 5
        Data collection and data processing - CryoSPARC live and CryoSPARC Conf. Room 1

        Conf. Room 1

        BHSS, Academia Sinica

        Speaker: Dr Chun-Hsiung Wang (Academia Sinica Cryo-EM Facility)
    • 12:00 PM
      Lunch 4F Recreation Hall

      4F Recreation Hall

      BHSS, Academia Sinica

    • Hands-on Session (3 groups, 5 trainees per group) Academia Sinica CryoEM Facility, B2, Interdisciplinary Sci. & Tech. Building

      Academia Sinica CryoEM Facility, B2, Interdisciplinary Sci. & Tech. Building

      • 6
        PART 1

        Group 1: Negative stain and Cryo-EM grid preparation (Dr. Yuan-Chih Chang)
        Group 2: Cryo-ET data collection demonstration on Talos (Dr. Chun-Hsiung Wang)
        Group 3: SPA data collection demonstration on Titan combined with CryoSPARC live (Dr. Irene Deli)

    • 2:00 PM
      Coffee Break
    • Hands-on Session (3 groups, 5 trainees per group) Academia Sinica CryoEM Facility, B2, Interdisciplinary Sci. & Tech. Building

      Academia Sinica CryoEM Facility, B2, Interdisciplinary Sci. & Tech. Building

      • 7
        PART 2

        Group 1: Cryo-ET data collection demonstration on Talos (Dr. Yuan-Chih Chang)
        Group 2: SPA data collection demonstration on Titan combined with CryoSPARC live (Dr. Chun-Hsiung Wang)
        Group 3: Negative stain and Cryo-EM grid preparation (Dr. Irene Deli)

    • 2:40 PM
      c
    • Hands-on Session (3 groups, 5 trainees per group) Academia Sinica CryoEM Facility, B2, Interdisciplinary Sci. & Tech. Building

      Academia Sinica CryoEM Facility, B2, Interdisciplinary Sci. & Tech. Building

      • 8
        PART 3

        Group 1: SPA data collection demonstration on Titan combined with CryoSPARC live (Dr. Yuan-Chih Chang)
        Group 2: Negative stain and Cryo-EM grid preparation (Dr. Chun-Hsiung Wang )
        Group 3: Cryo-ET data collection demonstration on Talos (Dr. Irene Deli)

    • 3:20 PM
      c
    • Hands-on Session (3 groups, 5 trainees per group) Academia Sinica CryoEM Facility, B2, Interdisciplinary Sci. & Tech. Building

      Academia Sinica CryoEM Facility, B2, Interdisciplinary Sci. & Tech. Building

      • 9
        Open Discussion and/or Consultation